An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation.
Identifieur interne : 000479 ( Main/Exploration ); précédent : 000478; suivant : 000480An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation.
Auteurs : Basdeo Bhagwat [Canada] ; Ming Chi ; Li Su ; Haifeng Tang ; Guiliang Tang ; Yu XiangSource :
- Journal of genetics and genomics = Yi chuan xue bao [ 1673-8527 ] ; 2013.
Descripteurs français
- KwdFr :
- ARN des plantes (génétique), Expression des gènes (MeSH), Ordre des gènes (MeSH), Plantes (génétique), Plantes (métabolisme), Solanum tuberosum (génétique), Solanum tuberosum (métabolisme), Tabac (génétique), Tabac (métabolisme), Transformation génétique (MeSH), Vecteurs génétiques (MeSH), Végétaux génétiquement modifiés (MeSH), microARN (génétique), microARN (métabolisme).
- MESH :
- génétique : ARN des plantes, Plantes, Solanum tuberosum, Tabac, microARN.
- métabolisme : Plantes, Solanum tuberosum, Tabac, microARN.
- Expression des gènes, Ordre des gènes, Transformation génétique, Vecteurs génétiques, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Gene Expression (MeSH), Gene Order (MeSH), Genetic Vectors (MeSH), MicroRNAs (genetics), MicroRNAs (metabolism), Plants (genetics), Plants (metabolism), Plants, Genetically Modified (MeSH), RNA, Plant (genetics), Solanum tuberosum (genetics), Solanum tuberosum (metabolism), Tobacco (genetics), Tobacco (metabolism), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : MicroRNAs, RNA, Plant.
- chemical , metabolism : MicroRNAs.
- genetics : Plants, Solanum tuberosum, Tobacco.
- metabolism : Plants, Solanum tuberosum, Tobacco.
- Gene Expression, Gene Order, Genetic Vectors, Plants, Genetically Modified, Transformation, Genetic.
Abstract
The utility of artificial microRNAs (amiRNAs) to induce loss of gene function has been reported for many plant species, but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable. In this study, expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation. A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments. Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs. This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.
DOI: 10.1016/j.jgg.2013.03.012
PubMed: 23706301
Affiliations:
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Le document en format XML
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last="Su">Li Su</name></author><author><name sortKey="Tang, Haifeng" sort="Tang, Haifeng" uniqKey="Tang H" first="Haifeng" last="Tang">Haifeng Tang</name></author><author><name sortKey="Tang, Guiliang" sort="Tang, Guiliang" uniqKey="Tang G" first="Guiliang" last="Tang">Guiliang Tang</name></author><author><name sortKey="Xiang, Yu" sort="Xiang, Yu" uniqKey="Xiang Y" first="Yu" last="Xiang">Yu Xiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23706301</idno><idno type="pmid">23706301</idno><idno type="doi">10.1016/j.jgg.2013.03.012</idno><idno type="wicri:Area/Main/Corpus">000457</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000457</idno><idno type="wicri:Area/Main/Curation">000457</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000457</idno><idno 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sortKey="Su, Li" sort="Su, Li" uniqKey="Su L" first="Li" last="Su">Li Su</name></author><author><name sortKey="Tang, Haifeng" sort="Tang, Haifeng" uniqKey="Tang H" first="Haifeng" last="Tang">Haifeng Tang</name></author><author><name sortKey="Tang, Guiliang" sort="Tang, Guiliang" uniqKey="Tang G" first="Guiliang" last="Tang">Guiliang Tang</name></author><author><name sortKey="Xiang, Yu" sort="Xiang, Yu" uniqKey="Xiang Y" first="Yu" last="Xiang">Yu Xiang</name></author></analytic><series><title level="j">Journal of genetics and genomics = Yi chuan xue bao</title><idno type="ISSN">1673-8527</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression (MeSH)</term><term>Gene Order (MeSH)</term><term>Genetic Vectors (MeSH)</term><term>MicroRNAs (genetics)</term><term>MicroRNAs (metabolism)</term><term>Plants (genetics)</term><term>Plants (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>RNA, Plant (genetics)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (metabolism)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term><term>Expression des gènes (MeSH)</term><term>Ordre des gènes (MeSH)</term><term>Plantes (génétique)</term><term>Plantes (métabolisme)</term><term>Solanum tuberosum (génétique)</term><term>Solanum tuberosum (métabolisme)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Transformation génétique (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>microARN (génétique)</term><term>microARN (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>MicroRNAs</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term><term>Plantes</term><term>Solanum tuberosum</term><term>Tabac</term><term>microARN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Plantes</term><term>Solanum tuberosum</term><term>Tabac</term><term>microARN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Gene Order</term><term>Genetic Vectors</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" 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In this study, expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation. A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments. Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs. This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23706301</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>05</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1673-8527</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>5</Issue><PubDate><Year>2013</Year><Month>May</Month><Day>20</Day></PubDate></JournalIssue><Title>Journal of genetics and genomics = Yi chuan xue bao</Title><ISOAbbreviation>J Genet Genomics</ISOAbbreviation></Journal><ArticleTitle>An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation.</ArticleTitle><Pagination><MedlinePgn>261-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jgg.2013.03.012</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1673-8527(13)00077-5</ELocationID><Abstract><AbstractText>The utility of artificial microRNAs (amiRNAs) to induce loss of gene function has been reported for many plant species, but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable. In this study, expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation. A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments. Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs. This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.</AbstractText><CopyrightInformation>Copyright © 2013. Published by Elsevier Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bhagwat</LastName><ForeName>Basdeo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, Pacific Agri-Food Research Center, Summerland, British Columbia V0H 1Z0, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chi</LastName><ForeName>Ming</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Haifeng</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Guiliang</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Yu</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal 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PubStatus="pubmed"><Year>2013</Year><Month>5</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>11</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23706301</ArticleId><ArticleId IdType="pii">S1673-8527(13)00077-5</ArticleId><ArticleId IdType="doi">10.1016/j.jgg.2013.03.012</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Chi, Ming" sort="Chi, Ming" uniqKey="Chi M" first="Ming" last="Chi">Ming Chi</name><name sortKey="Su, Li" sort="Su, Li" uniqKey="Su L" first="Li" last="Su">Li Su</name><name sortKey="Tang, Guiliang" sort="Tang, Guiliang" uniqKey="Tang G" first="Guiliang" last="Tang">Guiliang Tang</name><name sortKey="Tang, Haifeng" sort="Tang, Haifeng" uniqKey="Tang H" first="Haifeng" last="Tang">Haifeng Tang</name><name sortKey="Xiang, Yu" sort="Xiang, Yu" uniqKey="Xiang Y" first="Yu" last="Xiang">Yu Xiang</name></noCountry><country name="Canada"><noRegion><name sortKey="Bhagwat, Basdeo" sort="Bhagwat, Basdeo" uniqKey="Bhagwat B" first="Basdeo" last="Bhagwat">Basdeo Bhagwat</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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